Open AccessPaenibacillus apii sp. nov., a novel nifH gene-harbouring species isolated from the rhizospheres of vegetable plants grown in different regions of northern China
Author(s) -
Shuai Tong,
Liwei Wang,
YuChen Sun,
Mohammad Sayyar Khan,
Jun-lian Gao,
Jianguang Sun
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004447
Subject(s) - biology , 16s ribosomal rna , paenibacillus , phylogenetic tree , diamino acid , peptidoglycan , microbiology and biotechnology , botany , phylogenetics , diaminopimelic acid , strain (injury) , gene , actinobacteria , genetics , gene sequence , anatomy
Two nifH gene-harbouring bacterial strains were isolated from rhizospheres of different vegetable plants grown in different regions of northern PR China. The two strains possessed almost identical 16S rRNA gene sequences. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two strains were 99.21 and 93.6% respectively, suggesting they belong to one species. Based on 16S rRNA gene phylogeny, the two strains were clustered together with Paenibacillus rhizophilus 7197 T , Paenibacillus sabinae T27 T and Paenibacillus forsythiae T98 T , but on a separate branch. Novelty of the species was confirmed by ANI and dDDH comparisons between the type strain 7124 T and its closest relatives, since the obtained values were considerably below the proposed thresholds for the species delineation. The genome size of strain 7124 T was 5.40 Mb, comprising 5050 predicted genes with a DNA G+C content of 52.3 mol%. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids. The major cellular fatty acids were anteiso-C 15 : 0 (52.9%) and C 16 : 0 (23.4 %). Menaquinone-7 was reported as the major respiratory quinone. The diamino acid in the cell-wall peptidoglycan was found to be meso -diaminopimelic acid. Based on phylogenetic, genomic, chemotaxonomic and phenotypic data, the two isolates are considered to represent a novel species of the genus Paenibacillus , for which the name Paenibacillus apii sp. nov. is proposed, with 7124 T (=DSM 103172 T =CGMCC 1.15689 T ) as type strain.