Open AccessMycolicibacterium nivoides sp. nov isolated from a peat bog
Author(s) -
John L. Dahl,
Wayne Gatlin,
Phuong M. Tran,
Cody S. Sheik
Publication year - 2021
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004438
Subject(s) - biology , 16s ribosomal rna , strain (injury) , clade , bacteria , phylogenetic tree , phylogenetics , ribosomal rna , peat , botany , microbiology and biotechnology , gene , genetics , ecology , anatomy
A fast-growing, non-chromogenic, acid-fast-staining bacterium (DL90 T ) was isolated from a peat bog in northern Minnesota. On the basis of 16S rRNA gene sequence similarity (99.8 % identity with Mycolicibacterium septicum and 98 % with Mycolicibacterium peregrinum ) and chemotaxonomic data (fatty acid content), strain DL90 T represents a member of the genus Mycolicibacterium . Physiological tests (growth curves, biofilm formation, antibiotic sensitivity, colony morphologies and heat tolerance) and biochemical analysis (arylsulfatase activity and fatty acid profiles) distinguish DL90 T from its closest relative M. septicum . Phylogenomic reconstruction of the ‘ Fortuitium–Vaccae ’ clade, digital DNA–DNA hybridization (DDH) values of 61 %, and average nucleotide identity (ANI) values of approximately 95 % indicate that DL90 T is likely to be diverged from M. septicum . Thus, we propose that DL90 T represents a novel species, given the name Mycolicibacterium nivoides with the type strain being isolate DL90 T (=JCM 32796 T =NCCB 100660 T ).