Open AccessRoseomonas bella sp. nov., isolated from lake sediment
Author(s) -
Jinyu Zhang,
Xiaobing Jiang,
Di Zhu,
Xiaoman Wang,
ZongJun Du,
Da-Shuai Mu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004436
Subject(s) - biology , 16s ribosomal rna , coccus (insect) , strain (injury) , phosphatidylglycerol , microbiology and biotechnology , oxidase test , bacteria , botany , phosphatidylcholine , genetics , biochemistry , anatomy , phospholipid , enzyme , membrane
A Gram-stain-negative, non-motile, coccus-shaped, catalase- and oxidase-positive, facultatively anaerobic and pink-pigmented bacterium, designated strain CQN31 T , was isolated from sediment of Changqiaohai Lake, Yunnan Province, China. Growth occurred at 4–45 °C (optimum, 37 °C), at pH 6.5–9.5 (optimum, pH 8.0) and with 0–1 % (w/v) NaCl (optimum, 0 %). C 18 : 1 ω 7 c /C 18 : 1 ω 6 c and C 16 : 0 were the predominant fatty acids. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidyldimethylethanolamine (PME) and one unidentified aminolipid (AL) were the major polar lipids. The G+C content of the genomic DNA was 71.5 %. 16S rRNA gene sequence comparisons indicated that strain CQN31 T shared 96.8 % similarity with Roseomonas wooponensis JCM 19527 T and 95.9 % with R. terricola EM0302 T . Digital DNA–DNA hybridization values between strain CQN31 T and Roseomonas stagni DSM 19981 T , R. rosea DSM 14916 T and R. mucosa NCTC 13291 T were 21.0, 19.4 and 19.8 %, respectively. Average amino acid identity and average nucleotide identity values between strain CQN31 T and R. stagni DSM 19981 T , R. rosea DSM 14916 T and R. mucosa NCTC 13291 T were 73.7, 63.4 and 61.9 %, and 79.2, 77.1 and 77.5%, respectively. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain CQN31 T as a representative of a novel species in the genus Roseomonas , for which the name Roseomonas bella sp. nov. is proposed. The type strain is CQN31 T (=KCTC 62447 T =MCCC 1H00309 T ).