Open AccessFlaviflexus ciconiae sp. nov., isolated from the faeces of the oriental stork, Ciconia boyciana
Author(s) -
Jae Yun Lee,
Woorim Kang,
Pil Soo Kim,
So Yeon Lee,
Na-Ri Shin,
Hojun Sung,
Jun-Young Lee,
Ji Hyun Yun,
Yun Seok Jeong,
Jeong Eun Han,
Mi Ja Jung,
Dong Wook Hyun,
Hyun Sik Kim,
Euon Jung Tak,
JinWoo Bae
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004435
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , peptidoglycan , diamino acid , microbiology and biotechnology , ribosomal rna , bacteria , biochemistry , gene , gene sequence , genetics , anatomy
A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48 T , was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48 T was observed at 30–37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48 T was closely related to the genus Flaviflexus , with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2 T and Flaviflexus huanghaiensis H5 T , respectively. Strain H23T48 T possessed MK-9(H 4 ) as the major menaquinone and C 16 : 0 (42.4 %), C 18 : 1 ω 9 c (31.3 %) and C 14 : 0 (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was l- alanine, l- lysine, d- glutamic acid, l- aspartic acid and glycine. The genomic G+C content of strain H23T48 T is 59.5 mol% and the average nucleotide identity value between H23T48 T and F. salsibiostraticola KCT C33148 T (=EBR4-1-2 T ) is 75.5 %. Based on the obtained data, strain H23T48 T represents a novel species of the genus Flaviflexus , for which the name Flaviflexus ciconiae sp. nov. is proposed. The type strain is H23T48 T (=KCTC 49253 T =JCM 33282 T ).