Open AccessNocardia aurantiaca sp. nov., isolated from soil in Thailand
Author(s) -
Pawina Kanchanasin,
Masahiro Yuki,
Takuji Kudo,
Moriya Ohkuma,
Wongsakorn Phongsopitanun,
Somboon Tanasupawat
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004432
Subject(s) - biology , nocardia , 16s ribosomal rna , nocardiosis , actinomycetales , microbiology and biotechnology , strain (injury) , diamino acid , peptidoglycan , diaminopimelic acid , arabinose , streptomyces , bacteria , botany , cell wall , biochemistry , xylose , genetics , fermentation , anatomy , gene sequence
A novel actinomycete strain, CT2-14 T , belonging to the genus Nocardia , was isolated from a soil sample collected from Phichit Province, Thailand. The taxonomic position of the strain was characterized using a polyphasic approach. The strain grew at 15–40 °C (optimum, 28–37 °C), pH 6–11 (optimum, pH 6–8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate. Meso -diaminopimelic acid was detected in the cell-wall peptidoglycan. Ribose, arabinose and galactose were detected in its whole-cell hydrolysates. Mycolic acids were present. The strain contained C 16 : 0 , summed feature 3, C 17 : 0 10-methyl and C 18 : 1 ω 9 c as the major fatty acids and MK-8(H 4 ω-cycl) as the major menaquinone. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol mannosides. Strain CT2-14 T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307 T (98.4 %), Nocardia africana JCM 11438 T (98.2 %) and Nocardia kruczakiae JCM 13032 T (98.0 %). The draft genome of strain CT2-14 T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %. Based on the phylogenomic tree analysis, the strain was closely related to Nocardia niigatensis NBRC 100131 T . On the basis of polyphasic and genome analyses, strain CT2-14 T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp. nov. is proposed. The type strain is CT2-14 T (=JCM 33775 T =TISTR 2838 T ).