Open AccessMyroides albus sp. nov., isolated from the gut of plastic-eating larvae of the coleopteran insect Zophobas atratus
Author(s) -
Mengli Xia,
Lin Hu,
YiXin Huo,
Yu Yang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004429
Subject(s) - biology , 16s ribosomal rna , housekeeping gene , phylogenetic tree , lineage (genetic) , genus , strain (injury) , gene , phylogenetics , genetics , microbiology and biotechnology , zoology , anatomy , gene expression
A bacterial strain, BIT-d1 T , was isolated from the gut of plastic-eating larvae of the coleopteran insect Zophobas atratus . Its taxonomic position was analysed using a polyphasic approach. Cells were white-pigmented, Gram-stain-negative, non-motile, long rods without flagella. The 16S rRNA gene sequence (1401 bp) of strain BIT-d1 T showed highest similarity (98.0%) to Myroides pelagicus SM1 T and 96.6~92.6 % similarity to the other species of the genus Myroides . The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of six housekeeping genes ( gyrB , dnaK , tuf , murG , atpA and glyA ) and genome sequences, placed strain BIT-d1 T in a separate lineage among the genus Myroides , family Flavobacteriaceae . The major isoprenoid quinone was menaquinone-6 (MK-6) and the major fatty acids were C 15 : 0 iso, C 17 : 0 iso 3-OH and summed feature 9 (comprising iso-C 17 : 1 ω 9 c and/or C 16 : 0 10-methyl), which were similar to other members in the genus Myroides. In silico DNA–DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests exhibited the genotypic and phenotypic differentiation of strain BIT-d1 T from the other members of the genus Myroides . Therefore, strain BIT-d1 T is considered to represent a novel species within the genus Myroides , for which the name Myroides albus sp. nov is proposed. The type strain is BIT-d1 T (=CGMCC 1.17043 T =KCTC 72447 T ).