Open AccessMicrolunatus elymi sp. nov., a novel actinobacterium isolated from rhizospheric soil of the wild plant Elymus tsukushiensis
Author(s) -
JinSoo Son,
Soo-Yeong Lee,
Ye-Ji Hwang,
JaeHo Shin,
SaYoul Ghim
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004428
Subject(s) - biology , peptidoglycan , 16s ribosomal rna , diamino acid , diaminopimelic acid , phylogenetic tree , genomic dna , microbiology and biotechnology , strain (injury) , botany , bacteria , gene , cell wall , biochemistry , genetics , gene sequence , anatomy
An actinobacterial strain, designated KUDC0627 T , was isolated from rhizospheric soil that contained Elymus tsukushiensis on the Dokdo Islands, Republic of Korea. Cells were Gram-stain-positive, facultative anaerobic, non-motile and non-endospore-forming cocci. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KUDC0627 T belongs to the genus Microlunatus and is most closely related to Microlunatus soli DSM 21800 T (98.5 %), Microlunatus endophyticus DSM 100019 T (97.7 %) and Microlunatus ginsengisoli Gsoil 633 T (96.5 %). The average nucleotide identity scores and average amino acid identity values were all below the 95.0 % cut-off point. In silico DNA–DNA hybridization, using the Genome-to-Genome Distance Calculator, estimated that there is 22.3 % DNA relatedness between KUDC0627 T and M. soli DSM 21800 T . The genomic DNA G+C content was 66.9 mol%. The major menaquinone was MK-9(H 4 ) and the major diagnostic diamino acid in the cell-wall peptidoglycan was ll -diaminopimelic acid. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and unidentified lipids. The major cellular fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 and iso-C 16 : 0 . Based on phenotypic, chemotaxonomic, and phylogenetic data, strain KUDC0627 T (=KCTC 39853 T =JCM 32702 T ) represents a novel species, for which the name Microlunatus elymi sp. nov. is proposed.