Open AccessGracilibacillus oryzae sp. nov., isolated from rice seeds
Author(s) -
Shanwen He,
Xing Wang,
Hebao Guo,
Jigang Han,
Kyu Kyu Thin,
Jieying Gao,
Liang Ma,
Xiaoxia Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004427
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phosphatidylglycerol , genomic dna , phylogenetic tree , botany , microbiology and biotechnology , bacteria , ribosomal rna , gene , phospholipid , biochemistry , genetics , membrane , phosphatidylcholine , anatomy
A Gram-stain-positive, facultatively anaerobic, endospore-forming bacterium, designated strain TD8 T , was isolated from surface-sterilized rice seeds ( Oryza sativa L.). Phylogenetic analysis of the 16S rRNA gene indicated that strain TD8 T should be placed within the genus Gracilibacillus (95.2–99.0 % sequence similarity); it exhibited highest similarities to Gracilibacillus ureilyticus CGMCC 1.7727 T (99.0 %), ‘ Gracilibacillus xinjiangensis ’ CGMCC 1.12449 T (98.9 %) and Gracilibacillus dipsosauri CGMCC 1.3642 T (97.5 %). Chemotaxonomic analysis showed that menaquinone-7 (MK-7) was the major isoprenoid quinone. Diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid were the major cellular polar lipids, and the major fatty acids were anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 , C 16 : 0 and iso-C 16 : 0 , which supported the allocation of the strain to the genus Gracilibacillus . The digital DNA–DNA hybridization value between strain TD8 T and Gracilibacillus ureilyticus CGMCC 1.7727 T was lower than 70 % (22.60 %), and the average nucleotide identity score was 79.54±5.09 %, suggesting that strain TD8 T represented a novel species in the genus Gracilibacillus . The genomic DNA G+C content was 37.5 %. Based on physiological and biochemical characteristics and genotypic data, strain TD8 T represents a novel species of the genus Gracilibacillus , for which the name Gracilibacillus oryzae sp. nov. is proposed. The type strain is TD8 T (=ACCC 61556 T =CICC 24889 T =JCM 33537 T ).