Gracilibacillus oryzae sp. nov., isolated from rice seeds

A Gram-stain-positive, facultatively anaerobic, endospore-forming bacterium, designated strain TD8 T , was isolated from surface-sterilized rice seeds ( Oryza sativa L.). Phylogenetic analysis of the 16S rRNA gene indicated that strain TD8 T should be placed within the genus Gracilibacillus (95.2–99.0 % sequence similarity); it exhibited highest similarities to Gracilibacillus ureilyticus CGMCC 1.7727 T (99.0 %), ‘ Gracilibacillus xinjiangensis ’ CGMCC 1.12449 T (98.9 %) and Gracilibacillus dipsosauri CGMCC 1.3642 T (97.5 %). Chemotaxonomic analysis showed that menaquinone-7 (MK-7) was the major isoprenoid quinone. Diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid were the major cellular polar lipids, and the major fatty acids were anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 , C 16 : 0 and iso-C 16 : 0 , which supported the allocation of the strain to the genus Gracilibacillus . The digital DNA–DNA hybridization value between strain TD8 T and Gracilibacillus ureilyticus CGMCC 1.7727 T was lower than 70 % (22.60 %), and the average nucleotide identity score was 79.54±5.09 %, suggesting that strain TD8 T represented a novel species in the genus Gracilibacillus . The genomic DNA G+C content was 37.5 %. Based on physiological and biochemical characteristics and genotypic data, strain TD8 T represents a novel species of the genus Gracilibacillus , for which the name Gracilibacillus oryzae sp. nov. is proposed. The type strain is TD8 T (=ACCC 61556 T =CICC 24889 T =JCM 33537 T ).