Nonomuraea antri sp. nov., an actinomycete isolated from cave soil in Thailand

A novel actinobacterium, designated strain NN258 T , was isolated from a cave soil sample collected from a karst cave at Khao No-Khao Kaeo, Nakhon Sawan province, Thailand. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Nonomuraea . Strain NN258 T showed the highest 16S rRNA gene sequence similarity values to Nonomuraea candida HMC10 T , Nonomuraea mesophila 6K102 T , Nonomuraea rubra DSM 43768 T , Nonomuraea diastatica KC712 T and Nonomuraea helvata IFO 14681 T . The strain formed an extensively branched substrate and aerial mycelia. The whole-cell hydrolysates contained meso -diaminopimelic acid as the diagnostic diamino acid, with glucose, madurose, mannose and ribose as the whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphotidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, two unidentified phospholipids, three unidentified sugar-containing phosphoaminolipids, an unidentified glycolipid and two unidentified lipids. The predominant menaquinone was MK-9(H 4 ), with minor amounts of MK-9(H 0 ), MK-9(H 2 ) and MK-9(H 6 ). Major cellular fatty acids (>10%) were iso-C 16 : 0 and 10-methyl-C 17 : 0 . The G+C content of the genomic DNA was 71.0 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain NN258 T and the reference strains were 79.9–80.9 % and 26.1–27.0 %, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strain NN258 T represents a novel species of the genus Nonomuraea , for which the name Nonomuraea antri sp. nov. is proposed. The type strain is NN258 T (=TBRC 11478 T =NBRC 114269 T ).