Open AccessAgriterribacter humi gen. nov., sp. nov., a novel bacterium of the family Chitinophagaceae isolated from soil of a farming field
Author(s) -
Jae-Chan Lee,
Kyung-Sook Whang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004397
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , bacteria , genome size , ribosomal rna , fatty acid , botany , phylogenetics , microbiology and biotechnology , gene , genome , genetics , biochemistry , anatomy
A Gram-stain-negative bacterium, designated strain YJ03 T , was isolated from a spinach farming field soil at Shinan in Korea. Strain YJ03 T was found to be an aerobic, non-motile and non-spore-forming bacterium which can grow at 10–33 °C (optimum, 25–28 °C), at pH 6.6–9.5 (optimum, pH 7.0–7.5) and at salinities of 0–1.0 % (w/v) NaCl (optimum, 0 % NaCl). Sequence similarities of the 16S rRNA gene of strain YJ03 T with the closely related relatives were in the range 93.9–92.2 %, and the results of phylogenomic analysis indicated that strain YJ03 T was clearly separated from species of the genera in the family Chitinophagaceae , showing average nucleotide identity values of 68.8–64.3 %. The predominant isoprenoid quinone was identified as MK-7 and the major fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and an unidentified fatty acid with an equivalent chain-length of 13.565. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, four unidentified aminolipids and six unidentified lipids. The G+C content of the genome was determined to be 41.8 mol%. On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences in this study, strain YJ03 T is considered to represent a novel species of a new genus in the family Chitinophagaceae , for which the name Agriterribacter humi gen. nov., sp. nov., is proposed. The type strain of Agriterribacter humi is YJ03 T (=KACC 19548 T =NBRC 113195 T ).