Open AccessRhodovarius crocodyli sp. nov., isolated from a crocodile pond
Author(s) -
Wen-Ming Chen,
Yi-Ru Xie,
Der-Shyan Sheu,
Jyh-Ming Tsai,
Shih-Yi Sheu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004396
Subject(s) - biology , 16s ribosomal rna , spermidine , phylogenetic tree , genomic dna , strain (injury) , microbiology and biotechnology , gene , genetics , biochemistry , anatomy , enzyme
Bacterial strain CCP-6 T , isolated from a freshwater pond in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain CCP-6 T is affiliated with species in the genus Rhodovarius . Strain CCP-6 T was most closely related to Rhodovarius lipocyclicus CCUG 44693 T with a 98.9% 16S rRNA gene sequence similarity. Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped and formed light pink-coloured colonies. Optimal growth occurred at 30 °C, pH 6 and in the absence of NaCl. The major fatty acids of strain CCP-6 T were C 18 : 1 ω 7 c , C 16 : 0 and C 19 : 0 cyclo ω 8 c . The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, three unidentified aminophospholipids and an unidentified phospholipid. The predominant polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content of the genomic DNA was 69.3 mol%. Strain CCP-6 T showed 85.8% average nucleotide identity and 14.5% digital DNA–DNA hybridization identity with Rhodovarius lipocyclicus CCUG 44693 T . On the basis of the genotypic, chemotaxonomic and phenotypic data, strain CCP-6 T represents a novel species in the genus Rhodovarius , for which the name Rhodovarius crocodyli sp. nov. is proposed. The type strain is CCP-6 T (=BCRC 81095 T =LMG 30310 T =KCTC 62188 T ).