Open AccessMetabacillus sediminilitoris sp. nov., a marine bacterium isolated from a tidal sediment
Author(s) -
Haiyan Mao,
Yuli Wei,
Yuxin Gao,
Jiahao Pei,
Yan Zhang,
Jiasong Fang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004392
Subject(s) - biology , 16s ribosomal rna , strain (injury) , microbiology and biotechnology , phylogenetic tree , peptidoglycan , bacteria , diaminopimelic acid , glycolipid , gene , genetics , biochemistry , anatomy
A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17 T , was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically. The strain could grow at 16–47 °C (optimum 37 °C), at pH 6.0–10.0 (optimum 6.0) and with 1–7% (w/v) NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17 T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211 T (98.6 %), followed by Metabacillus halosaccharovorans E33 T (97.9 %), Metabacillus crassostreae JSM 100118 T (97.7 %), Metabacillus niabensis 4T19 T (97.7 %) and Metabacillus malikii NCCP-662 T (97.5 %). 16S rRNA gene sequence similarities between strain DSL-17 T and other members of the genus Metabacillus were below 96.6 %. The sole respiratory quinone was MK-7. Strain DSL-17 T had a cell-wall peptidoglycan based on meso -diaminopimelic acid. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified glycolipids and six unidentified lipids. The strain had iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 14 : 0 , C 16 : 0 and iso-C 16 : 0 as major fatty acids. The G+C content of the genomic DNA was 35.7 mol%. The combined genotypic and phenotypic data indicated that strain DSL-17 T represents a novel species of the genus Metabacillus , for which the name Metabacillus sediminilitoris sp. nov. is proposed The type strain is DSL-17 T (=MCCC 1K03777 T =DSM 109843 T ).