Open AccessGulosibacter macacae sp. nov., a novel actinobacterium isolated from Macaca mulatta faeces
Author(s) -
Gui-Ding Li,
Qinyuan Li,
Xiu Chen,
Long-Qian Jiang,
Kun Zhang,
De-Feng An,
Lang Lei,
Yi Jiang,
Li Han,
ChengLin Jiang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004389
Subject(s) - biology , feces , microbiology and biotechnology
A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1 T , was isolated from the faeces of Macaca mulatta . Strain YIM 102482-1 T grew optimally at 30–37 °C, at pH 8.0 and in the presence of 1.0–3.0% (w/v) NaCl. Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1 T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706 T (97.6 %), Gulosibacter bifidus NBRC 103089 T (97.6 %), Gulosibacter chungangensis KCTC 13959 T (96.4 %) and Gulosibacter molinativorax DSM 13485 T (96.0 %), respectively. Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1 T formed a distinct branch with all type strains of the genus Gulosibacter . The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C 15 : 0 , iso-C 16 : 0 and C 16 : 0 , respectively. The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan. The dominant polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipid. The DNA G+C content of YIM 102482-1 T was 63.0 mol%. Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1 T represents a novel species of the genus Gulosibacter , for which the name Gulosibacter macacae sp. nov. is proposed. The type strain is YIM 102482-1 T (=DSM 102156 T =CCTCC AB 2016023 T ).