Open AccessNonomuraea nitratireducens sp. nov., a new actinobacterium isolated from Suaeda australis Moq. rhizosphere
Author(s) -
Yixin Ou,
Yong Sheng,
Xiaojing Hu,
Dongjin Leng,
Jinhong Huang,
Zejun Hu,
Linquan Bai,
Zixin Deng,
Qianjin Kang,
YingYing Wu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004377
Subject(s) - biology , rhizosphere , 16s ribosomal rna , phylogenetic tree , diaminopimelic acid , strain (injury) , botany , genome , diamino acid , microbiology and biotechnology , peptidoglycan , genome size , whole genome sequencing , gene , bacteria , cell wall , biochemistry , genetics , gene sequence , anatomy
A novel actinomycete, designated WYY166 T , was isolated from the rhizosphere of Suaeda australis Moq. collected in Dongfang, PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on its 16S rRNA gene referred strain WYY166 T to the genus Nonomuraea , and it was most closely related to the type strains Nonomuraea candida HMC10 T , Nonomuraea turkmeniaca DSM 43926 T , Nonomuraea maritima NBRC 106687 T and Nonomuraea polychroma DSM 43925 T (98.35, 97.60, 97.36 and 97.30% sequence similarity, respectively). Genome sequencing revealed a genome size of 11.27 Mbp and a G+C content of 71.10 mol%. The genome average nucleotide identity (ANI) values and the digital DNA - DNA hybridization (dDDH) values between strain WYY166 T and the other species of the genus were found to be low (ANI 81.63~85.23 %, dDDH 23.6~31.6 %), suggesting that it represented a new species. The physiological evaluation showed that it had remarkable nitrate reduction activity. The whole-cell hydrolysates contained meso -diaminopimelic acid and madurose. The N -acyl type of muramic acid was acetyl. The major menaquinones were MK-9 (H 4 ) (86.9 %) and MK-9 (H 2 ) (13.1 %). The predominant fatty acids were iso-C 16 : 0 (53.2 %), 10-methyl C 17 : 0 (10.7 %), C 17 : 1 ω 6 c (8.3 %) and iso-C 16 : 1 h (7.3 %). These physiological, biochemical and chemotaxonomic data suggested that strain WYY166 T should be classified as representing a novel species of the genus Nonomuraea , for which the name Nonomuraea nitratireducens sp. nov. is proposed. The type strain is WYY166 T (=MCCC 1K03779 T =KCTC 49343 T ).