Open AccessStreptomyces lycii sp. nov., an endogenous actinomycete isolated from Lycium ruthenicum
Author(s) -
Li-Ran Ma,
Hong Zeng,
Zhan-Feng Xia,
Xiaoxia Luo,
Li-Li Zhang,
ChuanXing Wan
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004372
Subject(s) - biology , 16s ribosomal rna , rpob , streptomyces , housekeeping gene , phylogenetic tree , actinomycetales , microbiology and biotechnology , strain (injury) , sequence analysis , gene , bacteria , genetics , gene expression , anatomy
A novel endogenous actinobacteria strain, designated TRM 66187 T , was isolated from Lycium ruthenicum sampled at Alar, Xinjiang, Northwest PR China, and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 66187 T with the genus Streptomyces . The whole-cell sugar pattern of TRM 66187 T consisted of galactose, glucose and ribose. The predominant menaquinones were MK-9(H 4 ) and MK-9(H 6 ). Major cellular fatty acids were iso-C 14:0 , iso-C 15:0 , anteiso-C 15:0 and anteiso-C 16:0 . The detected polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified polar lipids. The G+C content of strain TRM 66187 T was 71.8 mol%. Results of phylogenetic analysis showed that strain TRM 66187 T had 98.48% 16S rRNA gene sequence similarity to the closest described species Streptomyces qinglanensis DSM 42035 T . The average nucleotide identity value between strain TRM 66187 T and the closest related strain Streptomyces qinglanensis DSM 42035 T was calculated to be 77.2%. The digital DNA–DNA hybridization value between them was 22.4%. Multilocus sequence analyses based on five housekeeping genes ( atpD , gyrB , recA , rpoB and trpB ) also indicated that strain TRM 66187 T should be assigned to the genus Streptomyces . On the basis of evidence from this polyphasic study, strain TRM 66187 T should be designated as representing a novel species of the genus Streptomyces , for which the name Streptomyces lycii sp. nov. is proposed. The type strain is TRM 66187 T (=LMG 31493 T =CCTCC AA 2018094 T ).