Open AccessAgrococcus sediminis sp. nov., an actinobacterium isolated from lake sediment
Author(s) -
Jianhang Qu,
Yi-Fei Yue,
Hailong Tian,
Jia Zhou,
Bang-Bang Yang,
Lei Deng,
Lingbo Qu,
Linfeng Wang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004371
Subject(s) - biology , microbiology and biotechnology , ecology
A novel strain, designated NS18 T , was isolated from sediment sampled at Taihu Lake, PR China. Cells of the isolate were spherical, aerobic, non-motile, Gram-stain-positive and non-endospore-forming. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18 T clustered in a clade of the genus Agrococcus . Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612 T with 98.2 % 16S rRNA gene sequence similarity. The complete genome of NS18 T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain NS18 T and A. lahaulensis DSM 17612 T based on their whole genomes were 85.1 and 28.7 %, respectively. The major fatty acids were anteiso-C 17 : 0 and anteiso-C 15 : 0 . The predominant menaquinones were MK11 and MK12. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids. The components of the peptidoglycan were Ala, Gly, Asp, Thr and DAB. The whole-cell sugars contained rhamnose, ribose, xylose and glucose. According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18 T (=NBRC 113859 T =MCCC 1K03759 T ) represents a novel species, for which the name Agrococcus sediminis sp. nov is proposed.