Open AccessIdeonella livida sp. nov., isolated from a freshwater lake
Author(s) -
Wen-Ming Chen,
Li-Chu Chen,
Der-Shyan Sheu,
Jyh-Ming Tsai,
Shih-Yi Sheu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004363
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , phosphatidylglycerol , gene , phylogenetics , lineage (genetic) , genomic dna , microbiology and biotechnology , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
A novel bacterial strain, designated TBM-1 T , isolated from a freshwater lake in Taiwan, was characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain TBM-1 T formed a phylogenetic lineage in the genus Ideonella . Analysis of 16S rRNA gene sequences showed that strain TBM-1 T was most closely related to Ideonella dechloratans CCUG 30898 T with 98.4 % sequence similarity. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain TBM-1 T and closely related strains of the genus Ideonella were 74.4–77.5 %, 69.7–75.4 % and 19.8–21.8 %, respectively, supporting that strain TBM-1 T represents a novel species of the genus Ideonella . Cells were Gram-stain-negative, motile by means of a single polar flagellum, rod-shaped and formed blue colonies. Optimal growth occurred at 30 °C, pH 6 and 0 % NaCl. The predominant fatty acids of strain TBM-1 T were summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), C 18 : 1 ω7 c and C 16 : 0 . The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two uncharacterized aminophospholipids and two uncharacterized phospholipids. The main polyamine was putrescine. The major isoprenoid quinone was Q-8. The estimated genome size was 5.26 Mb, with an average G+C content of 70.0 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TBM-1 T should be classified in a novel species of the genus Ideonella , for which the name Ideonella livida sp. nov. is proposed. The type strain is TBM-1 T (=BCRC 81199 T =LMG 31339 T ).