Open AccessCraterilacuibacter sinensis gen. nov. sp. nov., isolated from a crater lake in China
Author(s) -
Zi-Xuan Liu,
Dorji Phurbu,
HongCan Liu,
YuGuang Zhou,
Aihua Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004353
Subject(s) - biology , china , crater lake , impact crater , taxonomy (biology) , botany , ecology , archaeology , astrobiology , history
Two bacterial strains, designated B2N2-7 T and B2N2-12, were isolated from Buteha crater lake in the Greater Khingan Mountain of China. The two strains were Gram-stain-negative, non-spore-forming, motile with a single polar flagellum, short rod-shaped bacteria. They were catalase- and oxidase-positive. Optimal growth occurred at 20–25 ℃, at pH 7.5–8.0 and with 0–1.0 % (w/v) NaCl. Based on phylogenomic analysis, strains B2N2-7 T and B2N2-12 were assigned to the family Neisseriaceae , and their 16S rRNA gene sequences showed the highest similarities to that of Aquitalea denitrificans 5YN1-3 T (<94.2 %). The predominant cellular fatty acids were C 16 : 0 and summed feature 3 (comprising C 16 : 1 ω 7c /C 16 : 1 ω 6 c ). The major respiratory quinone was ubiquinone 8 (Q-8). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL) and some unidentified lipids (L). The genomic DNA G+C content of strain B2N2-7 T was 59.4 mol% according to the genomic sequencing result. Based on the phylogenetic, genotypic and chemotaxonomic analyses, the two strains are proposed to represent a novel species of a new genus in the family Neisseriaceae , named Craterilacuibacter sinensis gen. nov., sp. nov. The type strain of Craterilacuibacter sinensis is B2N2-7 T (=CGMCC 1.17189 T =KCTC 73735 T ); B2N2-12 (=CGMCC 1.17190=KCTC 72734) is a second strain of the species.