Open AccessAquitalea aquatilis sp. nov., isolated from Jungwon waterfall
Author(s) -
Hien T. T. Ngo,
HongYong Kim,
Huan Trinh,
TaeHoo Yi
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004351
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , bacteria , microbiology and biotechnology , botany , gene , genetics , anatomy
A Gram-stain-negative, facultative anaerobic, motile, short rods and yellow-pigmented bacterium, designated strain THG-DN7.12 T , was isolated from water collected at Jungwon waterfall on Yongmun mountain, Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN7.12 T was found to be most closely related to Aquitalea denitrificans 5YN1-3 T (98.9 % sequence similarity), Aquitalea magnusonii TRO-001DR8 T (98.7 %) and Aquitalea pelogenes P1297 T (98.0 %). The DNA–DNA relatedness between strain THG-DN7.12 T and its phylogenetically closest neighbours was below 70.0 %. The strain's DNA G+C content was 59.7 mol%. The major polar lipid was found to be phosphatidylethanolamine. Summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and C 16 : 0 were identified as the major fatty acids. Ubiquinone Q-8 was detected as the only respiratory quinone. These data supported the affiliation of strain THG-DN7.12 T to the genus Aquitalea . Strain THG-DN7.12 T was distinguished from related Aquitalea species by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name Aquitalea aquatilis sp. nov. is proposed, with THG-DN7.12 T as the type strain (=KACC 18847 T =CCTCC AB 2016185 T ).