Open AccessChitinophaga solisilvae sp. nov., isolated from forest soil
Author(s) -
Weiwei Ping,
Ying Zhang,
Huancheng Pang,
Jun Zhang,
Li Dai,
Yuyi Li,
Jianli Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004350
Subject(s) - biology , 16s ribosomal rna , genomic dna , strain (injury) , microbiology and biotechnology , soil microbiology , bacteria , botany , gene , genetics , anatomy
A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, O9 T , was isolated from a forest soil sample collected at Dai, Xishuangbanna, Yunnan Province, PR China. Strain O9 T grew optimally at pH 7.0, at 28‒30 °C and in the absence of NaCl. 16S rRNA gene sequence analysis placed strain O9 T within the genus Chitinophaga of the family Chitinophagaceae, with Chitinophaga terrae KP01 T (97.8 %), Chitinophaga jiangningensis JN53 T (97.7 %), Chitinophaga niastensis JS16-4 T (97.4 %), Chitinophaga qingshengii JN246 T (97.3 %) and Chitinophaga dinghuensis DHOC24 T (97.3 %) as its closest relatives. Strain O9 T hydrolysed casein, gelatin and Tween 80. It could not assimilate l -arabinose, l -rhamnose, sucrose, melibiose, gentiobiose or d -fructose as a carbon source. It was negative for esterase lipase (C8) and β -glucosidase. Phosphatidylethanolamine was the predominant polar lipid. The major respiratory quinone of strain O9 T was MK-7. Its major fatty acids were iso-C 15:0 (34.2 %), C 16:1 ω 5 c (20.9 %) and iso-C 17:0 3-OH (12.6 %). The genomic DNA G+C content of strain O9 T was 49.0 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain O9 T and that of Chitinophaga terrae KP01 T was 72.9%. The Genome-to-Genome Distance Calculator showed that DNA‒DNA hybridization values for strain O9 T and Chitinophaga terrae KP01 T were 13.6, 21.1 and 14.4%, respectively. Based on the polyphasic taxonomic data, strain O9 T represents a novel species of the genus Chitinophaga , for which the name Chitinophaga solisilvae sp. nov. is proposed. The type strain is O9 T (=CGMCC 1.12462 T =KCTC 32404 T ).