Streptomyces coryli sp. nov., isolated from hazelnut orchard soil

A novel actinobacteria, isolate A7024 T , was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 16S rRNA gene sequences indicated that isolate A7024 T was positioned within the members of the genus Streptomyces with the highest sequence similarity (97.7 %) to Streptomyces cadmiisoli ZFG47 T . The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024 T contained ll -diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H 6 ) and MK-9(H 4 ). The major cellular fatty acids were iso-C 16 : 0 , anteiso - C 15 : 0 and anteiso-C 17 : 0 . Strain A7024 T had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 ± 2.3% digital DNA–DNAhybridization and 76.94 % average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024 T represents a novel species of the genus of Streptomyces , for which the name Streptomyces coryli sp. nov. is proposed. The type strain is A7024 T (=DSM 42066 T =KCTC 29102 T =NRRL B-24888 T ).