Open AccessFundicoccus ignavus gen. nov., sp. nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk
Author(s) -
Annemarie Siebert,
Christopher Huptas,
Mareike Wenning,
Stephen W. Scherer,
Etienne V. Doll
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004344
Subject(s) - biology , phylogenetic tree , 16s ribosomal rna , peptidoglycan , type species , genus , phylogenetics , diamino acid , microbiology and biotechnology , glycolipid , strain (injury) , lineage (genetic) , cell wall , bacteria , gene , genetics , zoology , biochemistry , gene sequence , anatomy
Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk. Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937 T , WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813 T (93.7–94.1 %) and Eremococcus coleocola M1831/95/2 T (93.5 %) as most closely related type species. The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5–9.5. Growth was observed from 12 to 39 °C. Their cell-wall peptidoglycan belongs to the A1 α type ( l -Lys-direct) consisting of alanine, glutamic acid and lysine. The predominant fatty acids were C 16 : 1 ω9 c , C 16 : 0 and C 18 : 1 ω9 c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found. The G+C content of strain WS4937 T was 37.4 mol% with a genome size of ~3.0 Mb. Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed. The type species of the novel genus is Fundicoccus ignavus gen. nov., sp. nov. WS4937 T (=DSM 109652 T =LMG 31441 T ).