Open AccessDescription of Luteithermobacter gelatinilyticus gen. nov., sp. nov., and Paremcibacter congregatus gen. nov., comb. nov. via reclassification of the genus Emcibacter
Author(s) -
Mi-Jeong Park,
Teddy Namirimu,
SungHyun Yang,
Kae Kyoung Kwon
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004334
Subject(s) - biology , 16s ribosomal rna , strain (injury) , mesophile , thermophile , phosphatidylglycerol , microbiology and biotechnology , bacteria , phospholipid , biochemistry , anatomy , genetics , membrane , phosphatidylcholine
Strain MEBiC09520 T , which was isolated from a tidal sediment in Incheon, Korea, is a pale yellow, rod-shaped bacterium, cells of which are 0.4–0.5 µm in width and 1.5–2 µm in length. Strain MEBiC09520 T shared 95.17 and 92.57% 16S rRNA gene sequence similarity with Emcibacter nanhaiensis and E. congregatus , respectively. It grew optimally at pH 6.0, at 55 °C and with 2.5–3.5% (w/v) NaCl. Its polar lipid components included phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), an unidentified phospholipid (PL), three unidentified aminolipids (ALs) and two unidentified lipids (L). The fatty acids C 16:0 , C 19:0 cyclo ω8 c , C 14:0 2-OH and summed feature 8 (C 18:1 ω7 c and/or C 18:1 ω6 c ) were predominantly present in its cell wall. Strain MEBiC09520 T was thermophilic, while E. nanhaiensis and E. congregatus were mesophilic. Although E. nanhaiensis showed no nitrate reduction activity, MEBiC09520 T and E. congregatus showed a positive reaction. These strains differed in carbohydrate utilization. In particular, E. congregatus was able to thrive on various carbohydrate substrates as compared to the other strains. The average nucleotide identity value was 69.92% between strain MEBiC09520 T and E. congregatus ZYL T , 70.38% between E. congregatus ZYL T and E. nanhaiensis HTCJW17 T , and 72.83% between strain MEBiC09520 and E. nanhaiensis HTCJW17 T . Considering these differences, strain MEBiC09520 T (=KCCM 43320 T =MCCC 1K03920 T ) is suggested to represent and novel species of a new genus, Luteithermobacter gelatinilyticus gen. nov., sp. nov., and E. congregatus should be reclassified as Paremcibacter congregatus gen. nov., comb. nov.