Open AccessGenomic insights into a novel species Rhodoferax aquaticus sp. nov., isolated from freshwater
Author(s) -
Taihua Li,
Ya Zhuo,
Chun-Zhi Jin,
Xuewen Wu,
So-Ra Ko,
FengJie Jin,
ChiYong Ahn,
HeeMock Oh,
Hyung-Gwan Lee,
Long Jin
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004325
Subject(s) - biology , phylogenetic tree , 16s ribosomal rna , genomic dna , strain (injury) , gene , genus , botany , genome , phylogenetics , genetics , anatomy
A novel non-phototrophic member of the genus Rhodoferax was obtained from freshwater. The purpose of this study was to analyse the genome of a nonphototrophic strain and propose a new species based on its phylogenetic, genomic, physiological and chemotaxonomic characteristics. The results of phylogenetic analysis based on 16S rRNA gene sequences supports that the strain, designated Gr-4 T , has a close relationship to the genus Rhodoferax . The observed average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain Gr-4 T and its closest related strains were 72.3–74.6 % and 21.9–22.8 %, respectively. These values were much lower than the species separation thresholds for ANI or dDDH of 95–96 and 70 %, respectively, and in fact fall in the intergeneric range. Strain Gr-4 T does not contain RuBisCO-related genes, but does contain GS/GOGAT pathway-related genes enabling nitrate ammonification. A polyphasic study and a genomic-level investigation were done to establish the taxonomic status of strain Gr-4 T . Based on the phylogenetic, genomic and physiological differences, it is proposed that the isolate be classified to the genus Rhodoferax as Rhodoferax aquaticus sp. nov. with isolate Gr-4 T (=KCTC 32394 T =JCM 19166 T ) as the type strain.