Open AccessWenzhouxiangella limi sp. nov., isolated from a salt lake
Author(s) -
Xiaoyu Zhang,
Rui Zhang,
Jiacheng Wang,
Ting Zhang,
ZongJun Du
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004320
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , genomic dna , oxidase test , glycolipid , phosphatidylglycerol , microbiology and biotechnology , gene , botany , biochemistry , phospholipid , enzyme , anatomy , membrane , phosphatidylcholine
A novel Gram-stain-negative, strictly aerobic, gliding and rod-shaped bacterial strain, designated strain C33 T , was isolated from Yuncheng Salt Lake, Shanxi, PR China. Strain C33 T grows optimally at 37 °C, pH 7.5 and 5.0 % (w/v) NaCl. Cells of strain C33 T are 0.3–0.5 µm wide and 1.0–2.0 µm long, catalase-positive and oxidase-positive. The major cellular fatty acids are iso-C 15 : 0 and iso-C 16 : 0 . The sole respiratory quinone is Q-8. The major polar lipids include phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid, one unidentified glycolipid and four unidentified lipids. The results of phylogenetic analysis based on 16S rRNA gene sequences indicate that strain C33 T has the highest similarities to Wenzhouxiangella marina KCTC 42284 T (97.4 %), Wenzhouxiangella sediminis XDB06 T (96.5 %) and ‘ Wenzhouxiangella salilacus ’ MCCC 1K03442 T (95.2 %). The percentage of conserved proteins and average amino acid identity values between strain C33 T and its close related species are higher than the threshold for dividing genera, the average nucleotide identity and digital DNA–DNA hybridization values are well below the threshold limits for species delineation. The genomic DNA G+C content is 63.7 mol%. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain C33 T is considered to represent a novel species of the genus Wenzhouxiangella , for which the name Wenzhouxiangella limi sp. nov. is proposed. The type strain is C33 T (=MCCC 1H00413 T =KCTC 72874 T ).