Open AccessPrevotella vespertina sp. nov., isolated from an abscess of a hospital patient
Author(s) -
Michael Buhl,
Matthias Marschal
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004316
Subject(s) - biology , prevotella , microbiology and biotechnology , abscess , bacteria , paleontology , genetics
A strain of an obligately anaerobic, Gram-stain-negative rod-shaped bacterium is described by phenotypical, biochemical and genotypical characterization. This strain A2879 T was isolated from an abscess swab of a patient sampled during routine care at hospital. Phylogenetic analyses (full-length 16S rRNA gene and whole-genome sequence) revealed the strain to belong to the genus Prevotella , but to be distant from recognized species, with the closest relationship to Prevotella veroralis . Unambiguous identification also proved possible by MALDI-TOF MS. The genomic DNA G+C content was 41.5 mol%. Strain A2879 T was moderately saccharolytic and proteolytic. The most abundant cellular long-chain fatty acids were anteiso-C 15 : 0 and iso-C 15 : 0 . In view of these data, strain A2879 T is considered to represent a novel species within the genus Prevotella , for which the name Prevotella vespertina sp. nov. is proposed. The type strain is A2879 T (=DSM 108027 T =CCOS 1233 T =CCUG72808 T ). As this strain has been isolated from a clinical sample, it is considered relevant for human medicine and health in general, and in particular for the fields of clinical microbiology and infectious diseases. This description will enable routine and research laboratories alike to easily identify the novel taxon, allowing its role in the context of human health and disease or microbiota to be further elucidated.