Open AccessMuricauda ochracea sp. nov., isolated from a tidal flat in the Republic of Korea
Author(s) -
DongJun Kim,
Yeonjae Yoo,
Jong Seong Khim,
Dongmin Yang,
Duleepa Pathiraja,
In Geol Choi,
Jae Jin Kim
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004312
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , bacteria , genomic dna , botany , microbiology and biotechnology , gene , genetics , anatomy
A yellowish-brown-coloured bacterium, designated strain JGD-17 T , was isolated from a tidal flat of Janggu-do, Garorim bay, Taean-gun, Chungcheongbuk-do, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-flagellated and long-rod-shaped. Growth was observed at 20–45 °C (optimum, 25–30 °C), at pH 6.0–10.0 (9.0) and with 1–5 % (w/v) NaCl (1–3 %). Results of 16S rRNA gene sequence analysis indicated that strain JGD-17 T was closely related to Muricauda nanhaiensis SM1704 T (96.1 %), Muricauda olearia CL-SS4 T (95.0 %), Muricauda beolgyonensis BB-My12 T (94.9 %), Muricauda marina H19-56 T (94.7 %) and Muricauda indica 3PC125-7 T (94.5 %). The ranges of values for the average nucleotide identity and digital DNA–DNA hybridization analyses with related strains were 71.3–74.1 % and 16.9–18.2 %. The genomic DNA G+C content was 41.1 mol%. Phylogenetic analysis using the neighbour-joining method showed that strain JGD-17 T formed a clade with Muricauda nanhaiensis SM1704 T , Muricauda lutaonensis CC-HSB-11 T , Muricauda lutea CSW06 T and Muricauda pacifica SM027 T . The major fatty acids were iso-C 15 : 0 (26.9 %), iso-C 15 : 1 G (19.5 %) and iso-C 17 : 0 3-OH (12.7 %). The predominant respiratory quinone was menaquinone-6. The polar lipids were phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and two unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain JGD-17 T represents a novel species within the genus Muricauda , for which the name Muricauda ochracea sp. nov. is proposed. The type strain is JGD-17 T (=KCTC 72732 T =KACC 21486 T =JCM 33817 T ).