Open AccessGimesia benthica sp. nov., a planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean
Author(s) -
Jian Wang,
Chujin Ruan,
Lei Song,
Ang Li,
Yaxin Zhu,
Xiaowei Zheng,
Li Wang,
Zujun Lu,
Ying Huang,
Wei Du,
YuGuang Zhou,
Li Huang,
Xin Dai
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004301
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , genomic dna , flavobacteriaceae , bacteria , oxidase test , microbiology and biotechnology , bacteroidetes , botany , gene , biochemistry , genetics , anatomy , enzyme
A Gram-stain-negative, stalked, oval-shaped and budding bacterial strain, designated E7 T , was isolated from a deep-sea water sample collected from the Northwest Indian Ocean. The novel strain was strictly aerobic, and catalase- and oxidase-positive. It grew at 6–40 °C (optimum 30 °C) and pH 5.5–8.0 (optimum pH 7.0–7.5). The strain required 0.5–9.0 % (w/v) NaCl (optimum 3.0–5.0 %) for growth. Aesculin, starch, pectin and Tween 20 were hydrolysed. Based on 16S rRNA gene sequence analysis, strain E7 T showed the highest similarity with Gimesia maris DSM 8797 T (97.5 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain E7 T and G. maris DSM 8797 T were 78.0 and 19.3 %, respectively. The predominant cellular fatty acids of strain E7 T were C 16 : 0 and summed feature 3 (comprising C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ). The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PMME), phosphatidyldimethylethanolamine (PDME), phosphatidylcholine (PC) and diphosphatidylglycerol (DPG). The genomic DNA G+C content of strain E7 T was 52.8 mol%. On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain E7 T represents a novel species of the genus Gimesia , for which the name Gimesia benthica sp. nov. is proposed. The type strain is E7 T (=CGMCC 1.16119 T =KCTC 72737 T ).