Open AccessFrigidibacter oleivorans sp. nov., isolated from a deep well with oil reservoir water
Author(s) -
Yaxi Zhang,
FangLing Li,
Su-Chen Ma,
Guodi Zheng,
Wenfeng Chen,
Wenjun Li,
Lei Wang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004294
Subject(s) - biology , deep water , petroleum engineering , oceanography , engineering , geology
A Gram-stain-negative, rod-shaped bacterium, designated XJ4 T , was isolated from oil-contaminated water, collected from Xinjiang Province, north-west PR China (45° 1′ 27″ N, 85° 6′ 14″ E). Growth occurred at 20–45 °C (optimum, 30 °C) and pH 6.0–10.0 (optimum, pH 6.0–7.0). Strain XJ4 T could tolerate up to 7 % (w/v) NaCl and grow optimally in the absence of NaCl. Phylogenetic analysis based on comparative sequence analysis of 16S rRNA gene sequences indicated that strain XJ4 T belonged to the genus Frigidibacter , and that was closely related to Frigidibacter mobilis cai42 T (97.2 %), Frigidibacter albus SP32 T (97.0 %) and Rhodobacter aestuarii JA296 T (97.0 %). The average nucleotide identity values between XJ4 T and three type strains were 77.9, 77.6 and 71.9 %, respectively. The DNA G+C content of strain XJ4 T was 69.5 mol%. The sole respiratory quinone was Q-10. The major cellular fatty acid was summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ), C 18 : 0 and 11-methyl C 18 : 1 ω7 c . The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, an unidentified aminolipid and unidentified lipids. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain XJ4 T represents a novel species of the genus Frigidibacter , for which the name Frigidibacter oleivorans sp. nov. is proposed. The type strain is XJ4 T (=CGMCC 1.13778 T =LMG 30952 T ).