Open AccessDescription of four Apiotrichum and two Cutaneotrichosporon species isolated from guano samples from bat-inhabited caves in Japan
Author(s) -
Masako Takashima,
Sanae Kurakado,
Otomi Cho,
Ken Kikuchi,
Junta Sugiyama,
Takashi Sugita
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004277
Subject(s) - biology , basidiomycota , internal transcribed spacer , guano , phylogenetic tree , ribosomal rna , zoology , botany , yeast , cave , genus , holotype , gene , genetics , ecology
Four new yeast species belonging to the genus Apiotrichum and two new yeast species belonging to Cutaneotrichosporon are described for strains isolated from guano samples from bat-inhabited caves in Japan. In 2005, we reported these isolates as Trichosporon species based on sequence analyses of the D1/D2 domain of large subunit (LSU) rRNA genes according to available basidiomycetous yeast classification criteria; however, to date, they have not been officially published as new species with descriptions. Their phylogenetic positions have been reanalysed based on comparison of internal transcribed spacer (ITS) region sequences (including the 5.8S rRNA gene) and the D1/D2 domain of the LSU rRNA gene with those of known species; we confirmed clear separation from previously described species. Physiological and biochemical properties of the isolates also suggest their distinctiveness. Therefore, we describe Apiotrichum akiyoshidainum (holotype JCM 12595 T ), Apiotrichum chiropterorum (JCM 12594 T ), Apiotrichum coprophilum (JCM 12596 T ), Apiotrichum otae (JCM 12593 T ), Cutaneotrichosporon cavernicola (JCM 12590 T ) and Cutaneotrichosporon middelhovenii (JCM 12592 T ) as new species. C. cavernicola showed particularly distinctive morphology including large inflated anomalous cells on the hyphae and germination from the cells, although clear clamp connections on the hyphae were not confirmed. Further study is needed to elucidate the morph of this species.