Open AccessPhytoactinopolyspora mesophila sp. nov., isolated from a saline–alkaline soil
Author(s) -
Xue-Mei Feng,
Xue-Han Zhai,
Shaowei Liu,
Chun-Miao Wang,
ChengHang Sun,
Xue Zhou,
Yajie Zhang,
Xiaojun Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004274
Subject(s) - biology , 16s ribosomal rna , mycelium , botany , phylogenetic tree , halophile , monophyly , microbiology and biotechnology , strain (injury) , bacteria , clade , gene , biochemistry , genetics , anatomy
A novel moderately halophilic, filamentous actinobacterium, designated as XMNu-373 T , was isolated from a saline–alkaline soil sample collected from the Mongolia Plateau, Dongwu County, Inner Mongolia Autonomous Region, PR China. The isolate grew optimally at 28‒37 °C, pH 7.0‒8.0 and with 2–5 % (w/v) NaCl. The substrate mycelia fragmented into rod-like elements, and the white aerial mycelia formed spore chains at maturity. The predominant menaquinone was MK-9(H 4 ). The polar lipids were diphosphatidylglycerol, three unidentified phosphoglycolipids, an unidentified aminophospholipid, two phosphatidylinositol mannosides, four unidentified phospholipids, phosphatidylglycerol and two unidentified lipids. The major cellular fatty acids were iso-C 16 : 0 , anteiso-C 17 : 0 and anteiso-C 15 : 0 . The genomic DNA G+C content was 66.2 mol%. It shared high 16S rRNA gene sequence similarities to Phytoactinopolyspora halotolerans YIM 96448 T (96.1 %) and Phytoactinopolyspora endophytica EGI 60009 T (96.0 %). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain XMNu-373 T resided in the clade of family Jiangellaceae , and it formed a monophyletic branch distinct from four other recognized type species in the subclade of the genus Phytoactinopolyspora . On the basis of polyphasic taxonomic evidence, strain XMNu-373 T represents a novel species of the genus Phytoactinopolyspora , for which the name Phytoactinopolyspora mesophila sp. nov. is proposed. The type strain is XMNu-373 T (=JCM 33740 T =CGMCC 4.7654 T ).