Open AccessCupriavidus agavae sp. nov., a species isolated from Agave L. rhizosphere in northeast Mexico
Author(s) -
Ivan Arroyo-Herrera,
Jessica Maldonado-Hernández,
Fernando Uriel Rojas-Rojas,
Georgina Meza-Radilla,
Violeta LariosSerrato,
María-Soledad Vásquez-Murrieta,
William B. Whitman,
Paulina Estradade los Santos
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004263
Subject(s) - biology , rhizosphere , 16s ribosomal rna , cupriavidus necator , agave , botany , strain (injury) , bacteria , microbiology and biotechnology , genetics , anatomy , polyhydroxyalkanoates
During the isolation of bacteria from the Agave L. rhizosphere in northeast Mexico, four strains with similar BOX-PCR patterns were collected. The 16S rRNA gene sequences of all four strains were very similar to each other and that of the type strains of Cupriavidus metallidurans CH34 T (98.49 % sequence similarity) and Cupriavidus necator N-1 T (98.35 %). The genome of strain ASC-9842 T was sequenced and compared to those of other Cupriavidus species. ANIb and ANIm values with the most closely related species were lower than 95%, while the in silico DNA–DNA hybridization values were also much lower than 70 %, consistent with the proposal that they represent a novel species. This conclusion was supported by additional phenotypic and chemotaxonomic analyses. Therefore, the name Cupriavidus agavae sp. nov. is proposed with the type strain ASC-9842 T (=LMG 26414 T =CIP 110327 T ).