Open AccessTerrilactibacillus tamarindi sp. nov., isolated from bark of Tamarindus indica
Author(s) -
Engkarat Kingkaew,
Ratthanatda Nuhwa,
Jirabhorn Piluk,
Sitanan Thitiprasert,
Nuttha Thongchul,
Somboon Tanasupawat
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004261
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , diamino acid , phosphatidylglycerol , microbiology and biotechnology , bacteria , gene , biochemistry , phospholipid , gene sequence , genetics , membrane , phosphatidylcholine , anatomy
A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1 T , was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand. This strain produced d -lactic acid from glucose. It grew at 20–45 °C (optimum, 30 °C), pH 3.5–9 (optimum, pH 7.0) and in the presence of 1–4 % (w/v) NaCl. The cell-wall peptidoglycan contained meso -diaminopimelic acid (A1γ). The major isoprenoid quinone was menaquinone 7 (MK-7). Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid. The predominant cellular fatty acids were anteiso-C 17 : 0 , anteiso-C 15:0, and iso-C 16 : 0 when cultivated on GYP agar plates. The 16S rRNA gene sequence similarity between strain BCM23-1 T and Terrilactibacillus laevilacticus NK26-11 T was 98.3 %. The draft genome of BCM23-1 T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%. The values of ANIb, ANIm and digital DNA–DNA hybridization between strain BCM23-1 T and T. laevilacticus NK26-11 T were 89.9, 90.8 and 40.4 %, respectively. The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1 T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp. nov. is proposed. The type strain is BCM23-1 T (=LMG 31662 T =JCM 33748 T =TISTR 2841 T ).