Open AccessFlavobacterium alkalisoli sp. nov., isolated from rhizosphere soil of Suaeda salsa
Author(s) -
Liang Xu,
Haitao Wang,
Jinxin Zhang,
Hui Zhang,
Shuai Wang,
Junsong Sun
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004255
Subject(s) - biology , 16s ribosomal rna , rhizosphere , phylogenetic tree , ribosomal rna , gene , strain (injury) , housekeeping gene , botany , microbiology and biotechnology , bacteria , genetics , gene expression , anatomy
A Gram-negative, strictly aerobic, gliding motility, none-spore forming, yellow, rods bacterial strain, designated XS-5 T , was isolated from rhizosphere soil of Suaeda salsa , in Tumd Right Banner, Inner Mongolia, PR China. A phylogenetic tree based on the 16S rRNA gene sequences and the phylogenomic tree both showed that strain XS-5 T clustered with Flavobacterium beibuense F44-8 T (shared 97.2 % of 16S rRNA gene similarity) and Flavobacterium rakeshii FCS-5 T (97.6 %), and shared <96.0 % of 16S rRNA gene similarities with all other type strains. Strain XS-5 T contained MK-6 as the major respiratory quinone. Its major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid; and the major fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH, C 16 : 0 , iso-C 15 : 0 3-OH, Summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 ω 7c ), and Summed feature 9 (iso-C 17 : 1 ω 9 c and/or C 16 : 0 10-methyl). The genome consisted of a 3 985 855 bp circular chromosome, with a G+C content of 37.9 mol%, predicting 3616 coding sequences genes, 45 tRNA genes and three rRNA operons. The average nucleotide identity, amino acid identity and digital DNA–DNA hybridization values of strain XS-5 T to F. beibuense F44-8 T and F. rakeshii FCS-5 T were 79.2 and 79.2 %, 81.7 and 81.6 %, 22.3 and 22.2 %, respectively. The results of phylogenetic, physiological and biochemical tests allowed the discrimination of strain XS-5 T from its phylogenetic relatives. Flavobacterium alkalisoli sp. nov. is therefore proposed with strain XS-5 T (=CGMCC 1.17077 T =KCTC 72459 T ) as the type strain.