Open AccessNonomuraea typhae sp. nov., an endophytic actinomycete isolated from the root of cattail pollen (Typha angustifolia L.)
Author(s) -
Chenghui Peng,
Xiaoxin Zhuang,
Zhiyan Wang,
Congting Gao,
Junwei Zhao,
Jia Song,
Chongxi Liu,
Yue Shen
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004249
Subject(s) - biology , botany , 16s ribosomal rna , phylogenetic tree , mycelium , strain (injury) , microbiology and biotechnology , bacteria , gene , genetics , anatomy
A novel endophytic actinomycete, designated strain p1410 T , was isolated from the root of cattail pollen ( Typha angustifolia L.) and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus Nonomuraea . It produced spiral chains of spores on aerial mycelium as well as forming a pseudosporangium. Whole-cell hydrolysates contained meso -diaminopimelic acid, glucose, ribose and madurose. The menaquinones detected were MK-9(H 2 ), MK-9(H 4 ) and MK-9(H 0 ). The major fatty acids were 10-methyl C 17 : 0 , iso-C 16 : 0 and C 17 : 0 . The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol mannoside and an unknown glycolipid. The DNA G+C content of the draft genome sequence, consisting of 11.4 Mbp, was 70.9 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed that strain p1410 T belongs to the genus Nonomuraea with the highest sequence similarity to Nonomuraea candida HMC10 T (98.6 %), but phylogenetically clustered with Nonomuraea endophytica YIM 65601 T (98.4 %) and Nonomuraea longicatena NRRL 15532 T (98.3 %). Based on its phenotypic characteristics, DNA–DNA relatedness and average nucleotide identity, the strain is considered to represent a novel species of the genus Nonomuraea , for which the name Nonomuraea typhae sp. nov. is proposed. The type strain is p1410 T (=CCTCC AA 2019044 T =JCM 33461 T ).