Open AccessSphingomonas suaedae sp. nov., a chitin-degrading strain isolated from rhizosphere soil of Suaeda salsa
Author(s) -
Hui Zhang,
Liang Xu,
Jinxin Zhang,
Junsong Sun
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004238
Subject(s) - biology , 16s ribosomal rna , rhizosphere , strain (injury) , botany , microbiology and biotechnology , phosphatidylglycerol , spermidine , bacteria , gene , biochemistry , phospholipid , genetics , anatomy , enzyme , membrane , phosphatidylcholine
A Gram-stain-negative, aerobic, chitin-degrading, motile bacterial strain with a single polar flagellum, designated XS-10 T , was isolated from saline soil sampled from the rhizosphere of Suaeda salsa , Tumd Right Banner, Inner Mongolia, PR China. Strain XS-10 T grew at 10–40 °C (optimum, 35 °C), pH 5.0–9.0 (optimum, pH 8.0) and 0–12.5% NaCl (optimum 2.0 %). The phylogenetic analysis based on both the 16S rRNA gene and the phylogenomic tree revealed that strain XS-10 T formed a clade with Sphingomonas turrisvirgatae MCT13 T and Sphingomonas koreensis JSS-26 T , sharing 98.4 and 97.5 % 16S rRNA gene similarities to S. koreensis JSS-26 T and S. turrisvirgatae MCT13 T , respectively. Spermidine and Q-10 were the major polyamine and the major respiratory quinone, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, two unidentified lipids and an unidentified aminophospholipid. The major fatty acids were summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω6 c ), C 16 : 0 and C 17 : 1 ω6 c . The genome of strain XS-10 T consisted of a 4 154 291 bp chromosome with a DNA G+C content of 65.5 mol%. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values of strain XS-10 T with S. turrisvirgatae MCT13 T and S. koreensis JSS-26 T were 77.8 and 78.6 %, 75.9 and 76.3 %, and 22.0 and 22.9 %, respectively. Based on the phylogenetic, phenotypic, and genotypic characteristics, strain XS-10 T is considered to represent a novel species of the genus Sphingomonas , for which the name Sphingomonas suaedae sp. nov. is proposed. The type strain is XS-10 T (=CGMCC 1.17078 T =JCM 33850 T ).