Open AccessCyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov., isolated from a saline lake
Author(s) -
Yonghong Liu,
Yuan-Guo Xie,
Li Li,
Hongchen Jiang,
Osama Abdalla Abdelshafy Mohamad,
Wael N. Hozzein,
BaoZhu Fang,
Wenjun Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004237
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , genus , genomic dna , genome , lineage (genetic) , gene , microbiology and biotechnology , botany , genetics
Two novel strains, designated SYSU L10167 T and SYSU L10180 T , were isolated from sediment sampled at Dabancheng saline lake in Xinjiang, PR China. A polyphasic approach was used to clarify the taxonomic positions of the two strains. Cells of the isolates were curved ring-like, horseshoe-shaped or rod-shaped, non-motile and non-spore-forming. Cells were Gram-stain-negative, aerobic, heterotrophic and rose-pigmented. The phylogenetic trees based on 16S rRNA gene sequences showed that strains SYSU L10167 T and SYSU L10180 T formed a distinct lineage within the genus Cyclobacterium . Strains SYSU L10167 T and SYSU L10180 T showed highest similarities to Cyclobacterium jeungdonense KCTC 23150 T (98.0 and 97.4%, respectively). Results of genomic analyses (including average nucleotide identity, digital DNA–DNA hybridization and the marker gene tree) and pan-genome analysis further confirmed that strains SYSU L10167 T and SYSU L10180 T were separate from each other and other species of the genus Cyclobacterium . The draft genomes of the isolates had sizes of 5.5–5.7 Mb and reected their major physiological capabilities. Based on phenotypic, physiological, chemotaxonomic and genotypic characterization, we propose that the isolates represent two novel species, for which the names Cyclobacterium salsum sp. nov. and Cyclobacterium roseum sp. nov. are proposed. The type strains of the species are SYSU L10167 T (=KCTC 72390 T =CGMCC 1.17521 T ) and SYSU L10180 T (=KCTC 72391 T =CGMCC 1.17278 T ).