Open AccessSphingomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench
Author(s) -
Shanshan Yang,
Xue-Gong Li,
Xiang Xiao,
Guang-jiao Zhuang,
Yu Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004235
Subject(s) - biology , sphingomonas , 16s ribosomal rna , strain (injury) , botany , phosphatidylglycerol , bacteria , phylogenetic tree , microbiology and biotechnology , gene , genetics , phosphatidylcholine , phospholipid , anatomy , membrane
A Gram-stain-negative, short rod-shaped, yellow bacterium (strain LMO-1 T ) was isolated from deep-sea sediment of the Mariana Trench, Challenger Deep. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain LMO-1 T belonged to genus Sphingomonas , with the highest sequence similarity to Sphingomonas formosensis CC-Nfb-2 T (96.3 %), followed by Sphingomonas prati W18RD T (96.1 %), Sphingomonas arantia 6P T (96.0 %) and Sphingomonas montana W16RD T (95.9 %). The predominant polar lipids were phosphatidylethanolamine, sphingoglycolipid, phosphatidylglycerol and phosphatidylcholine. The main cellular fatty acids were summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ), C 16 : 0 and C 14 : 0 2-OH. The major polyamine was sym -homospermidine and the predominant isoprenoid quinone was ubiquinone-10. The genome DNA G+C content of strain LMO-1 T was 69.2 mol%. The average nucleotide identity and DNA–DNA hybridization values between strain LMO-1 T and CC-Nfb-2 T were 75.9 and 20.5 %, respectively. Based on these data, LMO-1 T should be classified as representing a novel species of the genus Sphingomonas , for which the name Sphingomonas profundi sp. nov. is proposed. The type strain is LMO-1 T (=MCCC 1K04066 T =JCM 33666 T ).