Open AccessActinomyces wuliandei sp. nov., Corynebacterium liangguodongii sp. nov., Corynebacterium yudongzhengii sp. nov. and Oceanobacillus zhaokaii sp. nov., isolated from faeces of Tibetan antelope in the Qinghai-Tibet plateau of China
Author(s) -
Wentao Zhu,
Junqin Li,
Xiaoxia Wang,
Jing Yang,
Shan Lu,
XinHe Lai,
Dong Jin,
YuYuan Huang,
Sihui Zhang,
Ji Pu,
Jia-Rong Zhou,
Zhihong Ren,
Ying Huang,
Xiaomin Wu,
Jianguo Xu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004232
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , strain (injury) , corynebacterium , bacteria , genomic dna , gene , genetics , anatomy
Eight Gram-stain-positive, rod-shaped bacterial strains were isolated from faeces of Tibetan antelopes on the Tibet-Qinghai Plateau of China. Genomic sequence analysis showed that the strains belong to the genera Actinomyces (strains 299 T and 340), Corynebacterium (strains 2184 T , 2185, 2183 T and 2189) and Oceanobacillus (strains 160 T and 143), respectively, with a percentage of similarity for the 16S rRNA gene under the species threshold of 98.7 % except for strains 160 T and 143 with Oceanobacillus arenosus CAU 1183 T (98.8 %). The genome sizes (and genomic G+C contents) were 3.1 Mb (49.4 %), 2.5 Mb (64.9 %), 2.4 Mb (66.1 %) and 4.1 Mb (37.1 %) for the type strains 299 T , 2183 T , 2184 T and 160 T , respectively. Two sets of the overall genome relatedness index values between our isolates and their corresponding closely related species were under species thresholds (95 % for average nucleotide identity, and 70 % for digital DNA–DNA hybridization). These results, together with deeper genotypic, genomic, phenotypic and biochemical analyses, indicate that these eight isolates should be classified as representing four novel species. Strain 299 T (=CGMCC 1.16320 T =JCM 33611 T ) is proposed as representing Actinomyces wuliandei sp. nov.; strain 2184 T (=CGMCC 1.16417 T =DSM 106203 T ) is proposed as representing Corynebacterium liangguodongii sp. nov.; strain 2183 T (=CGMCC 1.16416 T =DSM 106264 T ) is proposed as representing Corynebacterium yudongzhengii sp. nov.; and strain 160 T (=CGMCC 1.16367 T =DSM 106186 T ) is proposed as representing Oceanobacillus zhaokaii sp. nov.