Open AccessGracilibacillus salitolerans sp. nov., a moderate halophile isolated from saline soil in Northwest China
Author(s) -
Lian Gan,
Xiaoguang Li,
Chengchang Jia,
Shihao Zhang,
Ruoshi Zhang,
Biyu Peng,
Yongqiang Tian
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004224
Subject(s) - biology , halophile , 16s ribosomal rna , peptidoglycan , strain (injury) , phylogenetic tree , genomic dna , microbiology and biotechnology , phosphatidylglycerol , phylogenetics , diaminopimelic acid , bacteria , diamino acid , sequence analysis , botany , gene , cell wall , biochemistry , phospholipid , genetics , gene sequence , anatomy , membrane , phosphatidylcholine
A moderately halophilic strain, designated SCU50 T , was recovered from a saline soil sample and characterized by a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain SCU50 T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8 T (98.1 % similarity) and Gracilibacillus orientalis XH-63 T (97.7 %). Genomic average nucleotide identity and digital DNA–DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species. The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%. The strain grew optimally at 10–15 % (w/v) NaCl, pH 6.5–7.5 and 25–30 °C. It contained anteiso-C 15 : 0 , iso-C 15 : 0 and anteiso-C 17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone. The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid. The cell-wall peptidoglycan type was A1γ based on meso -diaminopimelic acid. Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50 T should be assigned as representing a novel species within the genus Gracilibacillus . Thus, a novel taxon named Gracilibacillus salitolerans sp. nov. was first established, with SCU50 T (=CGMCC 1.17336 T =KCTC 43107 T ) as the type strain.