Open AccessMarinicella rhabdoformis sp. nov., isolated from coastal sediment
Author(s) -
Yan-Lin Zhong,
Xun-Ke Sun,
Jian-Gang Hui,
Hui-Ling Teng,
ZongJun Du
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004210
Subject(s) - biology , phylogenetic tree , strain (injury) , 16s ribosomal rna , phylogenetics , clade , genomic dna , psychrophile , genetics , bacteria , gene , anatomy
A Gram-stain-negative, rod-shaped, facultative anaerobic bacterium, designated strain 3539 T , was isolated from coastal sediment of Weihai, PR China. Optimal growth occurred at 28 °C, pH 7.5–8.0 and in the presence of 3.0 % (w/v) NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 3539 T formed a robust clade with members of the genus Marinicella and was closely related to Marinicella litoralis JCM 16154 T , Marinicella sediminis F2 T and Marinicella pacifica sw153 T with 97.7, 96.2 and 95.4 % sequence similarity, respectively. The average amino acid identity, percentage of conserved proteins, average nucleotide identity and digital DNA–DNA hybridization values between strain 3539 T and M. litoralis JCM 16154 T were 64.9, 68.3, 72.8 and 18.9 %, respectively. The genomic DNA G+C content of strain 3539 T was 42.0 mol%. The dominant respiratory quinone was ubiquinone-8, and the major fatty acids were iso-C 15 : 0 and summed feature 3 (C 16 : 1 ω7 c /C 16 : 1 ω6 c ). The polar lipids of strain 3539 T consisted of phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminophospholipid, one unidentified lipid and three unidentified phospholipids. Based on the combination of phylogenetic, phenotypic and chemotaxonomic data, strain 3539 T is considered to represent a novel species within the genus Marinicella in he family Alcanivoracaceae , for which the name Marinicella rhabdoformis sp. nov. is proposed. The type strain of the new species is 3539 T (=KCTC 72414 T =MCCC 1H00388 T ).