Open AccessFlavobacterium profundi sp. nov., isolated from a deep-sea seamount
Author(s) -
Qian Wang,
Sheng-Dong Cai,
Jie Liu,
De-Chao Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004205
Subject(s) - biology , seamount , flavobacterium , deep sea , zoology , oceanography , microbiology and biotechnology , fishery , bacteria , paleontology , pseudomonas , geology
The Gram-stain-negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP390 T , was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain TP390 T was related to the genus Flavobacterium and had highest 16S rRNA gene sequence identity with the type strain of Flavobacterium jejuense EC11 T (97.8 %). Sequence similarities to all other type strains of current species of the genus Flavobacterium were below 97 %. The predominant cellular fatty acids were iso-C 15 : 0 and iso-C 15 : 1 G. The quinone system for strain TP390 T comprised predominantly menaquinone MK-6 and the polar lipid profile contained phosphatidylethanolamine, four unknown aminolipids, one glycolipid and six unknown polar lipids. The genomic DNA G+C content of strain TP390 T was 31.2 mol%. In addition, the maximum values of in silico DNA–DNA hybridization ( is DDH) and average nucleotide identity (ANI) between strain TP390 T with F. jejuense KCTC 42149 T were 22.60 and 80.01% respectively. Combined data from phenotypic, phylogenetic, is DDH and ANI data demonstrated that the strain TP390 T is representative of a novel species of the genus Flavobacterium , for which we propose the name Flavobacterium profundi sp. nov. (type strain TP390 T =KACC 18559 T =CGMCC 1.15398 T ).