Open AccessGongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal)
Author(s) -
M. Rosário Martins,
C. Santos,
Célia Maria Gonçalves Soares,
Cledir Santos,
Nelson Lima
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004201
Subject(s) - biology , internal transcribed spacer , ribosomal dna , vineyard , botany , ribosomal rna , taxonomy (biology) , 16s ribosomal rna , mucorales , spacer dna , phylogenetics , bacteria , genetics , mucormycosis , gene , horticulture , medicine , pathology
This study describes a novel fungal species belonging to the genus Gongronella . During a previous work focusing on metalaxyl degradation by Mucorales strains, two isolates from vineyard soil samples collected in the Alentejo region, south Portugal, were identified as a putative novel species based on combined molecular and MALDI-TOF MS data. This new species is described here using a polyphasic approach that combines morphology, internal transcribed spacer of ribosomal DNA (ITS) and 28S ribosomal DNA (LSU) sequence data analysis and proteomic profiling by MALDI-TOF MS. Phenotypic and molecular data enabled this novel species to be clearly distinguished from other Gongronella species with results of combined ITS+LSU analysis showing that the Gongronella species is related to Gongronella butleri and Gongronella brasiliensis . Therefore, from the results of morphological and molecular analyses, isolates MUM 10.262 and MUM 10.263 seem to represent a new Gongronella species and the name Gongronella eborensis sp. nov. is proposed, with the ex-type strain MUM 10.262 (=CCMI 1100=CBS 128763).