Open AccessOleiliquidispirillum nitrogeniifigens gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from oil reservoir water
Author(s) -
FangLing Li,
Xiaotong Wang,
Jian-Jie Shan,
Shuai Li,
Yaxi Zhang,
Xizhe Li,
Dong-An Li,
Wenjun Li,
Lei Wang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004200
Subject(s) - biology , 16s ribosomal rna , rhodospirillaceae , phosphatidylglycerol , phylogenetic tree , strain (injury) , genomic dna , botany , bacteria , microbiology and biotechnology , phosphatidylcholine , gene , genetics , phospholipid , anatomy , membrane
A novel Gram-staining-negative, spiral-shaped bacterium, designated strain 64-1 T , was isolated from oil reservoir water collected from Liaohe oilfield, north-eastern China. Growth occurred at 15–55 °C and pH 6.0–10.0. The sole respiratory quinone was Q-10. The predominant cellular fatty acids were summed feature 8 (C 18 : 1 ω 7 c /C 18 : 1 ω 6 c ), C 16 : 0 and C 19 : 0 cyclo ω 8 c . The polar lipids consisted of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), an unidentified aminophospholipid (UAPL), an unidentified aminolipid (UAL) and two unidentified polar lipids (UPL). The genomic DNA G+C content of strain 64-1 T was 64.5 mol%. Strain 64-1 T shared the highest 16S rRNA gene sequence similarities with Phaeospirillum chandramohanii JA145 T (92.0 %) and Telmatospirillum siberiense 26-4b1 T (91.8 %). In the phylogenetic trees, the strain constituted a sub-cluster within the family Rhodospirillaceae . Based on the results of morphological, physiological, biochemical and phylogenetic analysis, strain 64-1 T represents a new species of a novel genus within the family Rhodospirillaceae , for which the name Oleiliquidispirillum nitrogeniifigens gen. nov., sp. nov. is proposed. The type strain is 64-1 T (=CGMCC 1.16798 T =LMG 31399 T ).