Open AccessExilibacterium tricleocarpae gen. nov., sp. nov., a marine bacterium from coralline algae Tricleocarpa sp.
Author(s) -
Guanghua Wang,
Xianxu Zheng,
Shuailiang Xu,
Ge Dang,
Hongfei Su,
Zhiheng Liao,
Biao Chen,
Wen Huang,
Jiayuan Liang,
Kefu Yu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004189
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , botany , bacteria , coralline algae , algae , microbiology and biotechnology , gene , biochemistry , genetics , anatomy
A Gram-stain-negative, non-spore-forming, aerobic, curved rod-shaped bacterium, designed strain R142 T , was isolated from a coralline algae Tricleocarpa sp. in the Beibu Gulf, China. Optimal growth occurred with 0–0.5 % (w/v) NaCl, at 25 °C and at pH 8. Global alignment based on 16S rRNA gene sequences indicated that strain R142 T shared 93.8 % similarity with its closest type strain, Pseudomaricurvus alkylphenolicus KU14G T . Phylogenetic analyses showed that strain R142 T forms a distinct branch alongside Maricurvus nonylphenolicus KU41E T , Pseudoteredinibacter isoporae SW-11 T , Pseudomaricurvus alkylphenolicus KU14G T , Pseudomaricurvus alcaniphilus MEBiC06469 T and Aestuariicella hydrocarbonica SM-6 T . The major polar lipids of strain R142 T were phosphatidylethanolamine and phosphatidylglycerol. The primary cellular fatty acids were C 16 : 0 , C 16 : 1 ω7 c , C 18 : 1 ω7 c , C 18 : 0 and C 14 : 0 . The genome DNA G+C ratio was 56.4 mol%. The only detected respiratory quinone was ubiquinone 8. The low 16S rRNA gene sequence similarity and differences in cellular fatty acids readily distinguished strain R142 T from all validly published type strains. Strain R142 T is therefore suggested to represent a novel species of a new genus, for which the name Exilibacterium tricleocarpae gen. nov., sp. nov. is proposed. The type strain of Exilibacterium tricleocarpae is R142 T (=MCCC 1K03816 T =KCTC 72138 T ).