Open AccessAmycolatopsis anabasis sp. nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior
Author(s) -
Hongfei Wang,
Xinyang Li,
Rui Gao,
Yuan-Guo Xie,
Min Xiao,
Qiuli Li,
Wenjun Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004184
Subject(s) - biology , 16s ribosomal rna , diamino acid , arabinose , microbiology and biotechnology , botany , strain (injury) , phylogenetic tree , peptidoglycan , diaminopimelic acid , gene , biochemistry , cell wall , xylose , gene sequence , anatomy , fermentation
A novel endophytic actinobacterium, designated strain EGI 650086 T , was isolated from the roots of Anabasis elatior (C.A.Mey.) Schischk. collected in Xinjiang, north-west China. The taxonomic position of the strain was investigated using a polyphasic taxonomic approach. Growth occurred at 15–40 °C, pH 6.0–8.0 and in the presence of 0–6 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086 T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717 T (97.1 %) and Amycolatopsis sacchari DSM 44468 T (97.0 %). Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %. The average nucleotide identity and DNA–DNA hybridization values between strain EGI 650086 T and the reference strains were 78.1–79.8 % and 22.1–23.0 %, respectively. The genome of strain EGI 650086 T was 10.9 Mb, with a DNA G+C content of 70.1 mol%. The diagnostic diamino acid in the peptidoglycan was meso -diaminopimelic acid. The major whole-cell sugars contained arabinose, galactose, glucose and ribose. The predominant menaquinones were MK-9 (H 4 ) and MK-9 (H 2 ). Major fatty acids were iso -C 16 : 0 and summed feature 4 ( iso -C 17 : 1 I and/or anteiso -C 17 : 1 B). The polar lipid profile of strain EGI 650086 T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid. Polyphasic taxonomic characteristics indicated that strain EGI 650086 T represents a novel species of the genus Amycolatopsis , for which the name Amycolatopsis anabasis sp. nov. is proposed. The type strain is EGI 650086 T (=KCTC 49044 T =CGMCC 4.7188 T ).