Open AccessCatellatospora sichuanensis sp. nov., a novel actinobacterium isolated from soil
Author(s) -
Chaolan Liu,
Yidong Guo,
Li Li,
Xiwei Wang,
Jiafu Lin,
Xinrong Wang,
Jianghua Li,
Yiwen Chu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004175
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , monophyly , clade , genus , phylogenetics , botany , bacteria , gene , genetics , anatomy
A novel actinobacterium, designated strain H14505 T , was isolated from a soil sample collected in Hong Yuan, Sichuan, southwest PR China. The temperature, pH and NaCl ranges for growth were determined to be 15–35 °C (optimum, 28 °C), 6.0–8.0 (optimum, pH 7.0) and 0–2 % (w/v; optimum without NaCl), respectively. The polar lipdis detected for strain H14505 T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, glycolipid and four unidentified lipids. The predominant menaquinones of strain H14505 T were MK-9(H 4 ) and MK-9(H 6 ), and the prevalent fatty acids (>10 %) were C 18 : 1 ω9 c , C 17 : 1 ω8 c , summed feature 5 (anteiso-C 18 : 0 / C 18 : 2 ω6,9 c ) and C 16 : 0 . Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences indicated that strain H14505 T showed high similarity to Catellatospora vulcania NEAU-JM1 T (99.0 %) and Catellatospora paridis NEAU-CL2 T (99.0 %), and formed a monophyletic clade within the the genus Catellatospora in the phylogenetic trees. However, the average nucleotide indentity and DNA–DNA hybridization values between strain H14505 T and closely related Catellatospora species showed that it belonged to a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of strain H14505 T from its closest relatives. Thus, it is proposed that strain H14505 T represents a novel species of the genus Catellatospora , for which the name Catellatospora sichuanensis sp. nov. is proposed. The type strain of Catellatospora sichuanensis is H14505 T (=JCM 32394 T =CICC 11042 T ).