Open AccessLysinibacillus antri sp. nov., isolated from cave soil
Author(s) -
Manik Prabhu Narsing Rao,
Zhou-Yan Dong,
Xueke Niu,
Kun Zhang,
Yingqian Kang,
Awalagaway Dhulappa,
Min Xiao,
Wenjun Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004169
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , genomic dna , phosphatidylglycerol , mannose , microbiology and biotechnology , galactose , cell wall , botany , bacteria , dna , biochemistry , phospholipid , genetics , anatomy , membrane , phosphatidylcholine
A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002 T , was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. SYSU K30002 T grew at 28–40 °C (optimum, 37 °C), at pH 5.0–8.0 (optimum, pH 7.0) and in the presence of 0–4 % (w/v) NaCl (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4 α (Lys–Asp). The cell-wall sugars of SYSU K30002 T were ribose, galactose and mannose, and MK-7 was the menaquinone. The major fatty acids were iso-C 15 : 0 , C 16 : 1 ω 7 c alcohol and iso-C 16 : 0 . The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The G+C content of the genomic DNA was 36.1 mol%. The average nucleotide identity values between SYSU K30002 T and its closest relatives were below the cut-off level (95–96 %) for species delineation. Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002 T represents a novel species of the genus Lysinibacillus , for which the name Lysinibacillusantri sp. nov. is proposed. The type strain is SYSU K30002 T (=KCTC 33955 T =CGMCC 1.13504 T ).