Open AccessKlebsiella indica sp. nov., isolated from the surface of a tomato
Author(s) -
Sukriti Gujarati,
Diptaraj Chaudhari,
Ashwini Hagir,
Mitesh Khairnar,
Yogesh S. Shouche,
Praveen Rahi
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004168
Subject(s) - biology , klebsiella , microbiology and biotechnology , enterobacteriaceae , botany , escherichia coli , gene , genetics
A novel bacterial strain, designated TOUT106 T , was isolated from the surface of a tomato. The cells were rod-shaped, Gram-negative, encapsulated and non-motile. Strain TOUT106 T grows best at 28 °C and pH 7.0 and can tolerate up to 2 % (w/v) NaCl. Based on 16S rRNA gene phylogeny, strain TOUT106 T was placed close to the Salmonella clade, with close similarity to Salmonella enterica subsp. arizonae strain NCTC 8297 T (98.42 %). Results of genome-based phylogenetic analysis revealed that strain TOUT106 T is placed well in the Klebsiella–Raoultella clade, by forming a distinct branch with Klebsiella michiganensis DSM25444 T , Klebsiella oxytoca NCTC132727 T , Klebsiella grimontii 06D021 T and Klebsiella pasteurii SB6412 T . The genomic DNA G+C content of strain TOUT106 T is 53.53 mol%. The average nucleotide identity values of TOUT106 T were less than 86.5 % with closely related members of the family Enterobacteriaceae . The major fatty acids of strain TOUT106 T were C 16 : 0 , C 17:0 cyclo, C 14:0 3OH/C 16:1 iso, C 14 : 0 , C 19:0 cyclo ω8 c , C 18:1 ω6 c /C 18:1 ω7 c , C 12 : 0 and C 16:1 ω7 c /C 16:1 ω6 c . Strain TOUT106 T showed differences in physiological, phenotypic and protein profiles by MALDI-TOF MS compared to its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain TOUT106 T could be distinguished from the recognized species of the genus Klebsiella . It is suggested to represent a novel species of this genus, for which the name Klebsiella indica sp. nov. is proposed. The type strain is TOUT106 T (=MCC 2901 T =KACC 21384 T =JCM 33718 T ).