Open AccessFrancisella salimarina sp. nov., isolated from coastal seawater
Author(s) -
Lianghui Li,
Hai-Min Luo,
Jun-Hui Feng,
Yu-Zhen Ming,
Min-Ling Zheng,
Guang-Yuan Deng,
Chen Cha,
Wenjun Li,
Min Xiao,
Pinghua Qu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004164
Subject(s) - biology , francisella , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , genus , genome size , phylogenetics , lineage (genetic) , ribosomal rna , botany , genome , gene , genetics , francisella tularensis , virulence
Four strains (SYSU SYW-1 T , SYW-2, SYW-3 and XLW-1) were isolated from seawater near the shore in Guangdong Province, China. Cells were Gram-stain-negative, aerobic, non-motile and non-spore-forming. Growth was observed at a temperature range of 16–40 °C (optimum, 32 °C), a pH range of 4–8 (optimum, pH 7) and in the presence of up to 10 % (w/v) NaCl. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and an unidentified phospholipid. The respiratory quinone was ubiquinone 8 (UQ-8), and the predominant fatty acids were C 18 : 0 3-OH, C 10 : 0 , C 14 : 0 and C 18 : 1 ω9 c . Comparison of 16S rRNA gene and genome sequences confirmed that these strains represented a novel member of the genus Francisella , with less than 98.8 % 16S rRNA gene sequence similarity and less than 95 % genomic average nucleotide identity to recognized Francisella species. The phylogenetic tree based on 16S rRNA gene sequences and the protein-concatamer tree based on a concatenation of 28 protein marker sequences both indicated that the strains clustered with ‘ Francisella salina ’ TX07-7308 and ‘ Francisella marina ’ E95-16, but formed a distinct lineage group among the other members of the genus Francisella . The DNA G+C contents of the four strains were determined to be 32.9, 32.7, 32.9 and 32.9 %, respectively (genome). On the basis of phenotypic and genotypic features, the strains are considered to represent a novel species of the genus Francisella , for which the name Francisella salimarina sp. nov. is proposed. The type strain is SYSU SYW-1 T (=CGMCC 1.17031 T =NBRC 113781 T ).